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. 2022 Jul 27;8(30):eabm5298. doi: 10.1126/sciadv.abm5298

Fig. 7. Electrophysiological characterization of AP-GluA2 KI.

Fig. 7.

(A) Overview of the experimental preparation, AAV stereotaxic injection in the dorsal hippocampus followed by intraperitoneal (IP) biotin injection (top), and whole-cell voltage-clamp electrophysiological recordings of CA1 pyramidal neurons in acute slices from adult mice to measure spontaneous or evoked synaptic responses by stimulation of Schaffer collaterals (bottom). (B) Representative current-voltage (I/V) plots of synaptic AMPA current amplitudes with fitting to a simple linear regression (WT in blue, KI in red, KI + eGFP AAV in gray, and KI + BirAER-eGFP AAV in orange). (C) Rectification index (RI) of synaptic AMPA currents. N ≥ 6. P ≥ 0.3020 (Kruskal-Wallis test; F = 4.135, P = 0.2473; Dunn’s post hoc test). (D) Ratio of synaptic NMDA and AMPA currents. N ≥ 6. P ≥ 0.9153 (Kruskal-Wallis test; F = 2.231, P = 0.5258; Dunn’s post hoc test). (E to G). Characterization of the amplitude (E), rise time (F), and decay time (G) of sEPSC. N ≥ 9. P ≥ 0.3840 (one-way ANOVA; F = 0.2545, P = 0.8576; F = 0.6686, P = 0.5764; F = 1.010, P = 0.3984; Tukey’s post hoc test). Representative traces and holding potentials are shown on the right of relevant panels. Error bars, SEM. See also fig. S19 for further synaptic and network characterization.