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. 2022 Jul 27;8(30):eabm5298. doi: 10.1126/sciadv.abm5298

Fig. 9. AMPAR immobilization by NA cross-linking blocks formation of contextual fear memories.

Fig. 9.

(A) Overview of the experimental design, stereotaxic injection of BirAER-eGFP or eGFP control AAV in the dorsal hippocampus and surgical implantation of the cannula used to deliver NA before fear acquisition, and postmortem histology to verify infusion sites (i and ii: basal freezing; iii: fear acquisition; iv: contextual fear control; v: cued fear expression; vi: contextual fear expression). (B) Representative tiled wide-field images of 60-μm frontal brain sections from KI mice injected with BirAER-eGFP or eGFP control AAV and infused with 8.33 μM NA conjugated to Texas Red by implanted cannula. Scale bars, 1000 and 250 μm. (C and D) Time course of freezing behavior expression observed during contextual fear memory testing (C) and upon CS presentation during cued fear memory testing (D). (E) Representative traces of mouse movements during the first 2 min of exposure to contexts A and B. (F) Mean freezing levels during contextual fear memory testing. N ≥ 9. *P ≤ 0.0391 and **P ≤ 0.0074 (Welch’s ANOVA; F = 12.13, P = 0.0001; Dunnett’s post hoc test). (G) Mean freezing levels during cued fear memory testing. P ≥ 0.7825 (one-way ANOVA; F = 0.6950, P = 0.5609; Dunnett’s post hoc test). See also figs. S21 and S22.