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. 2022 Jul 27;8(30):eabo0340. doi: 10.1126/sciadv.abo0340

Fig. 2. Muscle LONP1 regulates the response of adipose tissue and liver to HFD.

Fig. 2.

(A) Pictures of adipose pads from indicated mice fed CD or HFD. (B) Hematoxylin and eosin (H&E) staining of epididymal WAT (eWAT) and BAT. Scale bar, 50 μm. n = 5 to 9. (C) Masson’s trichrome staining in eWAT. Scale bar, 50 μm. n = 5 to 9. (D) Expression of genes [quantitative reverse transcription polymerase chain reaction (qRT-PCR)] in BAT from indicated mice fed CD or HFD. n = 6. (E) Western blot analysis of BAT from indicated mice. n = 4. (F) Quantification of UCP1/tubulin, NDUFB8/tubulin, TOM20/tubulin, and TIM23/tubulin in BAT from indicated mice fed HFD. n = 4. (G) UCP1 immunohistochemistry (IHC) staining in BAT from indicated mice fed CD or HFD. Scale bar, 50 μm. n = 4 to 5. (H) Mitochondrial respiration rates were determined from BAT of indicated genotypes. Succinate/rotenone (Suc/Rot)–stimulated, adenosine 5′-diphosphate (ADP)–dependent, and antimycin-induced respiration is shown. n = 5 to 8. (I) Expression of genes involved in UPRmt in BAT from indicated mice fed CD or HFD. n = 6. (J) Liver weight. n = 8 to 10. (K) H&E and Oil Red O staining of livers. Scale bar, 50 μm. n = 5 to 6. (L) Liver triglyceride levels. n = 6 to 8. Color legend for the panel: white, WT CD; gray, LONP1 mKO CD; orange, WT HFD; diagonal hatch, LONP1 mKO HFD. Values represent means ± SEM. *P < 0.05 versus corresponding WT controls; #P < 0.05 versus corresponding CD controls. Two-tailed unpaired Student’s t test (D, F, H, and I) or one-way ANOVA (J and L) was performed. AU, arbitrary units.