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. 2022 May 9;15(8):2292–2306. doi: 10.1111/1751-7915.14072

Table 1.

Yeast strains used in this study.

Strains Description Source
YS58 MATα flo1 ura3‐52 leu2‐3,112 his4‐519 trp1‐789 Teunissen et al. (1993)
YS58‐tHMGR YS58, Δhmg1::P TEF1 tHMG1‐T CYC1 This work
YS58‐HbHMGR YS58, Δhmg1::P TEF1 HbHMG1‐T CYC1 This work
YS58‐CrHMGR YS58, Δhmg1::P TEF1 CrHMG1‐T CYC1 This work
YS58‐YlHMGR YS58, Δhmg1::P TEF1 YlHMG1‐T CYC1 This work
YS58‐EfMvaE YS58, Δhmg1::P TEF1 EfmvaE‐T CYC1 This work
YS58‐EfHMGR YS58, Δhmg1::P TEF1 EfHMGR‐T CYC1 This work
YS58‐GAD1 YS58, Δadh2::P TEF2 GAD1‐T SUP4 This work
YS58‐PAN6 YS58, Δadh2::P TEF2 PAN6‐T SUP4 This work
YS58‐CAB1 YS58, Δadh2::P TEF2 CAB1‐T SUP4 This work
YS58‐GPC YS58, Δadh2::P TEF2 GAD1‐T SUP4 ‐P IRA1 PAN6‐T SUP4 ‐ P PGK1 CAB1‐T SUP4 This work
YS58‐ScE1G YS58 containing plasmid pYC‐SEGA This work
YS58‐PpE1G YS58 containing plasmid pYC‐PEGA This work
YS58‐HpE1G YS58 containing plasmid pYC‐HEGA This work
YS58‐PrE1G YS58 containing plasmid pYC‐XEGA This work
YS58‐PrE1 YS58 with replacement of wild‐type ERG1 promoter by P. rhodozyma ERG1 promoter This work
YEH‐56 S. cerevisiae with high sterol pathway activity He et al. (2000)
SQ‐3 YEH‐56 with overexpression of ERG10, ERG13, tHMG1, ERG12, ERG8, ERG19, IDI1, ERG20 and ERG9 Zhou et al. (2021)
SQ3‐EfHMGR SQ‐3, Δhmg1::P TEF1 EfHMGR‐T HMG1 This work
SQ3‐1 SQ‐3, Δcit2::P IRA1 EfHMGR‐T CIT2 This work
SQ3‐2 SQ3‐1, Δmls1::P IRA1 gapC‐T MLS1 This work
SQ3‐3 SQ3‐2, Δpdc1::P TEF2 CDC19‐linker‐PDC1‐T PDC1 This work
SQ3‐4 SQ3‐3, Δadh2::P TEF2 GAD1‐T SUP4 ‐P IRA1 PAN6‐T SUP4 ‐P PGK1 CAB1‐T ADH2 This work
SQ3‐5 SQ3‐4 with replacement of wild‐type ERG1 promoter by P. rhodozyma ERG1 promoter This work
SQ‐3‐CPS SQ‐3 containing plasmid pYE‐CPS This work
SQ3‐4‐CPS SQ3‐4 containing plasmid pYE‐CPS This work