Figure 2.
PCR assay of the cell pellets and supernatants from heat-killed (100°C, 10 minutes and 30 minutes) cells (8.5 log cells/mL) of (A) L. gasseri and (B) L. salivarius. Experimental conditions were as described in the Methods section. Lane M represents a 100-bp λ ladder. Lanes 1–5 represent the PCR results from pelleted cells (1 and 3) and cell-free supernatant (2 and 4), of cells heated for 10 minutes and 30 minutes, respectively. Lane 5 represents a negative control with sterilized H2O as the sample. PCR = polymerase chain reaction.