Skip to main content
. 2016 Nov 5;25(4):872–880. doi: 10.1016/j.jfda.2016.09.002

Figure 3.

Figure 3

Effects of yam extracts on (A) the protein expressions of caspase-3, caspase-8, caspase-9, and PARP-1, and (B) the release of Cyt. c in heart tissues of mice treated with DOX for 4 weeks. The protein levels of Cyt. c from Mito. (Lines 1–2) and Cyto. (Lines 3–4) fractions were determined by Western blotting. COX IV and β-actin, respectively, served as internal controls of Mito. and Cyto. fractions. The protein levels above the figure represent relative density of the bands normalized to COX IV or β-actin. The determined expression of the protein was subsequently quantified by densitometric analysis with that of control being 1.00-fold, as shown just below the gel data. Results are representative of at least three independent experiments. AE = ethanol extracts of D. alata; AW = water extracts of D. alata (Ta-Shan); Cyt. c = cytochrome c; Cyto. = cytosolic; DOX = doxorubicin; IL = interleukin; JE = ethanol extracts of D. japonica Thunb.; JW = water extracts of D. japonica Thunb.; Mito. = mitochondrial; PARP-1 = poly (ADP-ribose) polymerase-1; PE = ethanol extracts of D. purpurea; PW = water extracts of D. purpurea.