Table 1.
Lineage, resistance profile and mutations evidenced by susceptibility tests and WGS of isolates from Mozambique.
| N | MZB Isolate ID |
Lineage | MTBDR plus | MTBDR sl | Xpert | Phenotypic test | Whole genome sequencing | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mut R | Mut H | Mut Fq | Mut iSLD | R | R | H | H | Sm | Mut R | Mut H | Mut Z | Mut E | Mut Sm | Mut iSLD | Mut Fq | Mut PAS | |||
| 1 | 227 | 4.3 | S531L | WT, S315T2 (Kat G) | Yes | Yes | Yes | No | No | rpoB Ser450Leu | KatG Ser315Thr | ||||||||
| 2 | 243 | 1 | WT8, WT6 | WT2 (inhA) | No | No | No | No | No | embC Thr270lle, embB Glu378Ala | |||||||||
| 3 | 751 | 4.3 | WT, S315T2 (Kat G) | No | Yes | Yes | Yes | Yes | rpoB Val170Phe | KatG Ser315Thr | pncA Lys96Arg | embB Met306Val | rpsL Lys43Arg | ||||||
| 4 | 894 | 4.3 | WT, S315T2 (kat G) | No | No | Yes | No | Yes | KatG Ser315Thr | rpsL Lys43Arg | |||||||||
| 5 | 964 | 2.2 | WT7, H526D | Yes | Yes | No | No | No | rpoB His445Tyr | ||||||||||
| 6 | 2075 | 4.3 | No | No | Yes | No | Yes | KatG Ser315Thr | rpsL Lys43Arg | ||||||||||
| 7 | 2078 | 4.3 | WT, S315T2 (kat G) | No | Yes | Yes | Yes | Yes | rpoB Val170Phe | KatG Ser315Thr | pncA Lys96Arg | embB Met306Val | rpsL Lys43Arg | ||||||
| 8 | 2368 | 4.1.1.3 | WT8, S531L | WT, S315T2 (kat G), WT1, T8C (inhA) | WT2, A90V (gyr A) | WT2, C14T (eis) | Yes | Inv | Inv | Inv | Inv | rpoB Ser450Leu | KatG Ser315Thr | pncA Arg154Gly | embB Met306Ile | gyrA Ala90Val | |||
| 9 | 2721 | 4.3 | WT7 | WT, S315T2 (kat G) | WT2, A90V (gyr A) | Yes | Yes | Yes | Yes | Yes | rpoB His445Tyr | KatG Ser315Thr | pncA Leu19Arg | embB Gln497Arg | rpsL Lys43Arg | gyrA Ala90Val | |||
| 10 | 2937 | 3 | No | No | Yes | No | No | kasA Gly269Ser | embB Met306Ile | rpsL Lys88Arg | |||||||||
| 11 | 3033 | 4.3 | S531L | WT, S315T2 (kat G) | Yes | Yes | Yes | Yes | Yes | rpoB Ser450Leu | KatG Ser315Thr | embB Gln497Arg | rpsL Lys43Arg | ||||||
| 12 | 3150 | 1 | No | No | No | No | Yes | embC Thr270Ile, embB Glu378Ala | |||||||||||
| 13 | 3185 | 4.3 | WT7 | WT, S315T2 (kat G) | WT2, A90V (gyr A) | Yes | Yes | Yes | Yes | Yes | rpoB His445Tyr | KatG Ser315Thr | pncA Leu19Arg | embB Gln497Arg | rpsL Lys43Arg | gyrA Ala90Val | |||
Mut R: mutations in rpoB gene related to rifampicin resistance; Mut H: mutations related no isoniazid resistance.
Mut Fq: mutations related to fluoroquinolones resistance; Mut iSLD: mutations related to injectable second-line drugs resistance; WT: absence of wild-type band; Xpert R: rifampicin resistance detected by Xpert MTB/RIF; R: rifampicin.
H: isoniazid; E: ethambutol; Sm: streptomycin; Z: pyrazinamide; PAS: para-aminosalicylic acid; NV: not valid phenotypic test.