Figure 5.

PPARγ inhibitor alleviates the anticancer effect induced by stigmasterol. The cells were co-treated with high concentrations of stigmasterol and GW9662 at 20 and 40 μmol/L, respectively, to detect the regulation of cell viability and metabolism. (a) Oil red staining, magnification of 100x. (b) Free fatty acid detection. (c) ATP detection. (d) NADPH detection. (e) Cell colony formation assay. (f) qPCR was applied to detect the expression of cyclinD, CDK2, CDK4, and CDK6. (g–h) Western blot was applied to detect the expression of cyclinD, CDK2. CDK4, and CDK6. Compared with the control group, ^#p < 0.05; ^##p < 0.01.