FIGURE 1.

Two major trypanosome species were detected from naturally infected cows. (A) Representative light micrographs of Giemsa-stained cow blood sample smears infected with T. vivax (look the wavy shape and long flagellum). (B) Representative light micrograph of Giemsa-stained cow blood sample smears infected with T. congolense showing the less wavy shape and shorter in length flagellum. (C) Molecular confirmation of the two trypanosomes. ML 100 bp marker, Tc +ve control for T. congolense, Tb, T. brucei, and Tv, T. vivax, and N, negative control, C1–C20 cows blood sample. (D) The mean PCV of cow microscopically trypanosome negative and positive from field samples, n = 371.