Extended Data Fig. 3. Comparison of RNAseq expression profiles in spleen, lung and brain tissues from Adar1^−/mZα and Adar1^WT/mZα mice.

a–c, Transcriptional comparison of spleen (a), lung (b) and brain (c) tissues from Adar1^−/mZα and Adar1^WT/mZα littermate mice at P1. Heatmaps show differentially expressed genes between Adar1^−/mZα and Adar1^WT/mZα control mice. These were 1,594 (p ≤ 0.05, q ≤ 0.05, ≥ 2-fold-change), 657 (p ≤ 0.05, q ≤ 0.05, ≥ 2-fold-change), and 379 (p ≤ 0.05, ≥ 2-fold-change) genes for the spleen, lung and brain, respectively (Supplementary Data Table 2). Columns represent individual mice, hierarchically clustered according to differential gene expression. Tables show GO functional annotation of the genes found upregulated and downregulated in the comparison between the two genotypes in each organ, performed with g:Profiler (https://biit.cs.ut.ee/gprofiler). P values for differential expression analyses were calculated with Qlucore Omics Explorer using two-sided t-tests and with the q value for false discovery rates (FDR) set to 0.05. Calculation of q values was adjusted for multiple hypothesis testing using the Benjamini-Hochberg method. P values for pathway analyses were calculated with g:Profiler using hypergeometric distribution tests and adjusted for multiple hypothesis testing using the g:SCS (set counts and sizes) algorithm, integral to the g:Profiler server (https://biit.cs.ut.ee/gprofiler). For all genotypes, n = 5 for spleen and lung, n = 4 for brain.