Fig. 1.

Growth characteristics of different cell lines after infection with VSV-NDV. Cell growth (A), viability (B), pH (C), and TCID₅₀/mL (D) were compared between five cell lines. The medium was exchanged completely before infection. Cells were grown in baffled 125 mL shake flasks and infected at a viable cell concentration of 2 × 10.⁶ cells/mL with VSV-NDV at an MOI of 1E-2. The TCID₅₀/mL at 0 hpi was calculated empirically, corresponding to an MOI of 1E-2 (only yellow symbol is visible, as the same MOI was used for all infections). Values (except HEK293SF-F and HEK293SF-D cells) are reported as the mean of a biological duplicate with two independent shake flasks. Error bars represent the standard deviation of duplicates