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. 2022 Jun 29;106(13-16):4945–4961. doi: 10.1007/s00253-022-12027-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — VSV-NDV infection of four suspension cell lines at different MOIs ranging from 1E-5 to 1E-1 in 125-mL shake flasks. Infectious virus titers were calculated by TCID₅₀ assay and plotted against the time post-infection. The TCID₅₀/mL at 0 hpi was calculated empirically corresponding to the respective MOI. A AGE1.CR.pIX in CD-U7 medium, B AGE1.CR pIX in CD-U7 medium, C HEK293SF in PEM medium, and D BHK-21 in PEM medium. This screening experiment was carried out once. For AGE1.CR and AGE1.CR.pIX, additional MOI were tested to verify the possible beneficial impact