Fig. 7.
Time course of metabolites for BHK-21 and HEK293SF cells in a 1 L bioreactor. BHK-21 (A) and HEK293SF cells (B) were cultured in 350 mL supplemented PEM medium. After a cell growth phase of 96 h, cells were infected with VSV-NDV at an MOI of 0.0001 and 0.01, respectively, by adding a 350 mL of fresh PEMs medium. Glucose, lactate, ammonium, and glutamine concentrations were measured with the BioProfile® 100 Plus (Nova Biomedical, USA). The dotted vertical line indicates the time of infection; the gray area indicates the cell growth phase. Bioreactor runs were carried out as a single scouting experiment