Table 1.
Impact of the cell concentration and various CaCl2 concentrations at time of infection on the TCID50/mL titer
| Cell line | 5 Mio | 10 Mio | 0.3 mM CaCl2 | 0.5 mM CaCl2 | 0.9 mM CaCl2 | 1 mM CaCl2 |
|---|---|---|---|---|---|---|
| AGE1.CR.pIX | + | + | ~ | ~ | ~ | ~ |
| AGE1.CR | + | ~ | ~ | ~ | ~ | ~ |
| BHK-21 | + | + | ~ | ~ | ~ | ~ |
| HEK293 Freesytle | - - | - - | ~ | ~ | ~ | - |
| MDCK Smith-8 | + | ~ | n.d | ~ | n.d | ~ |
| MDCK Driving M | ~ | + | n.d | ~ | n.d | ~ |
Each condition was rated based on the achieved effect on the TCID50/mL titer compared to control infection at 2 × 10.6 cells/mL: + + : strong increase above 1 log, + : slight increase above 0.3 log, -: slight decrease below 0.3 log,—-: strong decrease above 1 log, ~ no change (within the 0.3 log dilution error of the TCID50 assay). n.d. = not determined