Table 2.
VSV-NDV-GFP production in suspension cell cultures. Yields and growth parameters of host cells after infection are shown. Cell concentration, virus decay time, and maximum infectious virus titers were measured in three independent shake flask cultivations
| Cell line | Media | Optimum MOI | Max. viable cell concentration (× 106 cells mL−1) | TCID50 mL−1 | CSVY* (virions cell−1) |
Time of max. titer (h) | 1 log decay time (h) |
|---|---|---|---|---|---|---|---|
| BHK-21sus | PEMs | 1 × 10−4 | 5.0 ± 1.0 | 8.8 × 108 ± 5.6 × 108 | 140 ± 84 | 36–42 | < 24 |
| HEK293SF | PEMs | 1 × 10−2 | 7.7 ± 1.0 | 8.3 × 107 ± 1.4 × 107 | 11 ± 3 | 96 | < 24 |
| AGE1.CR | CD-U7 | 1 × 10−5 | 4.3 ± 0.6 | 4.3 × 107± 2.2 × 107 | 11 ± 4 | 42–48 | < 12 |
| AGE1.CR.pIX | CD-U7 | 1 × 10−4 | 3.3 ± 0.2 | 5.4 × 106 ± 2.2 × 106 | 2 ± 1 | 48 | < 12 |
*CSVY, cell-specific virus yield