Skip to main content
. Author manuscript; available in PMC: 2023 Jul 25.
Published in final edited form as: Curr Biol. 2022 Jun 9;32(14):3016–3032.e3. doi: 10.1016/j.cub.2022.05.037

Figure 6. Effects of NDi1 on sensitivity to volatile anesthetics.

Figure 6.

A. EC50s for Loss of Righting Reflex (LORR) (isoflurane, EC50,LORR Ndi1(−) 0.73+/−0.03%; Ndi1(+) 0.95+/−0.08%; n=15; p<0.001) and (halothane, EC50,LORR Ndi1(−) 0.88+/−0.07%; Ndi1(+) 1.12+/−0.11%; n=15; p<0.001) and nonmovement to tail clamp (TC) (isoflurane, EC50,TC Ndi1(−) 1.31+/−0.08%; Ndi1(+) 1.58+/−0.12%; n=15; p<0.001) and (halothane, EC50,TC Ndi1(−) 1.27+/−0.09%; Ndi1(+) 1.54+/−0.11%; n=15; p<0.001) for wildtype and NDi1(+) mice. Left panel is the response to isoflurane, right panel is the response to halothane. Effect sizes are the percent change of the Ndi1 responses compared to the wildtype responses. B. EC50s for Loss of Righting Reflex (LORR) exposed to isoflurane (EC50,LORR Ndi1(−),Ndufs4(KO) 0.62+/−0.10%; Ndi1(+),Ndufs4(KO) 0.83+/−0.16%; n=7; p<0.001) and halothane (EC50,LORR Ndi1(−),Ndufs4(KO) 0.61+/−0.06%; Ndi1(+),Ndufs4(KO) 0.85+/−0.12%; n=7; p<0.001) and nonmovement to tail clamp (TC) for Ndufs4(KO) and NDi1;Ndufs4(KO) mice (isoflurane, EC50,TC Ndi1(−),Ndufs4(KO) 0.67+/−0.07%; Ndi1(+),Ndufs4(KO) 0.90+/−0.11%; n=7; p<0.001) and (halothane, EC50,TC Ndi1(−),Ndufs4(KO) 0.68+/−0.01%; Ndi1(+),Ndufs4(KO) 0.92+/−0.06%; n=7; p<0.001). Left panel is the response to isoflurane, right panel is the response to halothane. Effect sizes are the percent change of the NDi1;Ndufs4(KO) responses compared to the Ndufs4(KO) responses. C. NDi1 increases fEPSPs in the presence of isoflurane in hippocampal slices from both Ndufs4(KO) (left panel) and wildtype (right panel) mice. Hippocampal slices from Ndufs4(KO) and NDi1;Ndufs4(KO) (left panel) are exposed to 0.25mM isoflurane; those from wildtype and NDi1 (right panel) are exposed to 0.5mM isoflurane. NDi1-containing slices recover more quickly, and show pronounced long term potentiation compared to those without NDi1 (Ndufs4(KO): n=6, NDi1;Ndufs4(KO): n=12, wildtype: n = 6, NDi1: n = 12). Red marks at top indicate that NDi1 differs from wildtype and NDi1;Ndufs4(KO) differs from Ndufs4(KO), p<0.05 at all points.