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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Gastroenterology. 2022 May 10;163(2):449–465. doi: 10.1053/j.gastro.2022.05.007

Figure 5. DNMT1 is required for YAP1-TEAD-driven HC-to-ICC transformation.

Figure 5.

(A) Representative IHC showing DNMT1 induction in Akt-NICD-transfected HCs in the early stage of HC-to-BEC conversion at 2w post-injection. (B) Experimental design illustrating 5-Azacytidine treatment, plasmids used for HDTVI, mice used in study and time-points analyzed. Representative IHC of WT (C) or Sox9KO (D) livers showing Akt-NICD-transfected cells. (E) Yap1KO livers showing Yap1-deleted Akt-NICD-transfected cells with imperfect HC-to-biliary reprogramming (blue-dashed lines). 5-Azacytidine-treated (F), Dnmt1-silenced (G) or TEAD-inhibited livers (H) showing defective HC-to-BEC reprogramming. Akt-NICD-transfected cells (black- or red-dashed lines). Red arrows point to Akt-HA transduced cells. Black arrows point to Akt-HA transduced cells in DN-TEAD livers. Scale bars:100 μm.