Table 1.

Data collection parameters

	Aurein 3.3	Uperin 3.5
Microscope	FEI Titan Krios G3i
Voltage (kV)	300
Grids	Type R2/1 Quantifoil
Magnification	npEFTEM SA105000 x
C2 aperture size (μm)	70	50
Objective aperture size (μm)	No aperture	100
Pixel size (Å)	0.85 A/px
Total exposure (e-/Å2)	40
Exposure time (s)	2	3
Number of movie frames per exposure	40	45
Energy filter slit width (eV)	15	20
Data collection software	EPU 2.8.0.1256 Digital Micrograph 3.32.2403
Number of exposures per hole	5
Defocus range (μm)	−0.5, −1.0, −1.5, −2, −2.5	−1.0, −1.5, −2, −2.5, −3, −3.5, −4
Number of micrographs collected/used for final map	5514/482	11857/3297