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. 2022 Jul 27;13:4336. doi: 10.1038/s41467-022-31761-y

Fig. 3. High protein feeding promotes a pro-IgA cytokine environment, specifically APRIL in the lamina propria.

Fig. 3

Mice were fed on either a high-protein (HP), high-carbohydrate (HC), or high-fat (HF) diet for 6 weeks and intestinal ileal gene expression of a April (HP vs. HC p = <0.0001, HP vs. HF p = <0.0001), b Baff (HP vs. HF p = 0.0005, HC vs. HF p = <0.0135), c Tslp (HP vs. HF p = 0.0005, HC vs. HF p = 0.0082), d Il4 (HP vs. HF p = 0.0033), e Il10 (HC vs. HF p = 0.0481) and f Tgfb (HP vs. HC p = 0.0481, HP vs. HF p = 0.0214) was determined by qPCR (a–c, e n = 7 and n = 8 mice per diet for HP and HC/HF diet respectively, d n = 6 and n = 4 mice per diet for HP/HC and HF diet respectively, f; n = 7 and n = 8 mice per diet for HP/HC and HF diet respectively). Data are represented as mean ± SEM. Results represent n = 2 independent experiments. *p < 0.05, **<0.01, ***<0.001, ****<0.0001 by ordinary one-way ANOVA followed by Tukey’s multiple comparisons test.