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. 2022 Jul 14;10:930355. doi: 10.3389/fcell.2022.930355

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Copyright © 2022 Langlois-Lemay and D’Amours.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dynamic localization of Cdc5/Polo kinase at SPBs. G1: Cdc5 is absent from cells. S: Cdc5 enriches at the non-duplicated SPB. G2 to metaphase: Cdc5 decorates the nucleus and the nuclear surface of both SPBs. Early anaphase: Cdc5 concentration at the nuclear surface of both SPBs increases. Late anaphase: Cdc5 relocates from the inner to the outer surface of both SPBs (and bud neck) where it stimulates mitotic exit. Blue color represents enrichment of Cdc5. Color intensity represents Cdc5 concentration levels.