Table 1.
PCR primers used in the study
| Primers | Primer sequences (5’–3’) | Target species/gene | Amplicon size (bp) | Reference # |
|---|---|---|---|---|
| ITS1 | TCCGTAGGTGAACCTGCGG | Universal fungi/ITS | — | [15] |
| ITS4 | TCCTCCGCTTATTGATATGC | |||
| CAL-F | CAAGAAGATCATAACTCAAT | C. albicans/ERG11 | ~1,600 | [17] |
| CAL-R | CAGAACACTGAATCGAAAGA | |||
| CGL-F | ATGTCCACTGAAAACACTTCTTTGG | C. glabrata/ERG11 | 1,599 | [18] |
| CGL-R | GTACTTTTGTTCTGGATGTCTCTTTTC | |||
| CTR-F | GTTTTCTACTGGATCCCATG | C. tropicalis/ERG11 | ~1,200 | [19] |
| CTR-R | TACATCTGTGTCTACCACC | |||
| CPA-F | GCGATAAGAATGCGGCCGCATGGCATTAGTTGATTTAGC | C. parapsilosis/ERG11 | 1,569 | [20] |
| CPA-R | ATCGTACAGGCATGCTCAGATTACACATGTATCT |
Note: ITS is a target commonly used for fungal (including Candida) species identification by DNA sequencing.
Abbreviation: ITS, internal transcribed spacer.