FIG. 1.

DNA construct used to express the PHAC1 synthase of P. aeruginosa in S. cerevisiae. Only the portion of the construct containing the gene (open box) and regulatory elements (shaded box) is shown. The partial amino acid sequence of the C terminus of the PHA synthase-isocitrate lyase (ICL) fusion is indicated using the one-letter symbols, with the amino acids derived from the PHAC1 protein given in plain letters, novel amino acids created at the fusion junction italicized, and the last 34 amino acids derived from the B. napus isocitrate lyase underlined. CTA-Pr, CTA1 promoter; CTA-Tr, CTA1 terminator; E, EcoRI; H, HindIII.