FIG. 5.
Analysis of PHA inclusions in S. cerevisiae. The wild-type (A) or recombinant yeast expressing the PHA synthase gene (B to D) was used to inoculate media containing 0.1% glucose, 2% Pluronic-127, and 0.1% oleic acid and was grown for 4 days before being processed for TEM. Panels C and D are close-up views of panel B. Arrows indicate the presence of PHA inclusions within membrane-bound organelles. ob, oil body. Bars indicate 1 μm (A and B) and 0.5 μm (C and D).