Figure 1.

Using neutrophils as a positive control for MPO expression by flow cytometry, eosinophils were found to give a false positive signal. Splenocytes were stained with antibodies specific for CD19, CD11b, Ly6G, F4/80, Ly6C, MPO, and SiglecF and using flow cytometry, the CD19⁻ cells were gated out. CD11b⁺Ly6G⁺ neutrophils (A) were analyzed for MPO expression (B). CD11b^HiLy6G⁻ cells were analyzed for F4/80 and Ly6C expression (C) and F4/80⁺Ly6C^Lo MPO expression is shown (D). MPO^Hi cells were further analyzed for SSC^Hi and SiglecF expression to identify eosinophils (E). The percent positive cells in dot plot gates and the MFI of histograms is provided. Data shown are representative of three mice.