Figure 3.

Splenic monocyte/macrophage subsets differentially express MPO. Splenocytes were stained with antibodies specific for CD19, CD11b, Ly6C, Ly6G, F4/80, MPO, and SiglecF and using flow cytometry, CD19⁻ cells were analyzed for Ly6G and CD11b to identify double positive neutrophils and Ly6G⁻CD11b^Hi and Ly6G⁻CD11b^Lo cells (A). CD11b^HiLy6G⁻SiglecF⁻ cells were analyzed for F4/80 and Ly6C expression and four Subpop (I–IV) were identified (B). MPO expression in Subpop I F4/80⁺Ly6C^Hi (C), II Ly6C^LoF4/80⁺ (D), III Ly6C⁻F4/80⁻ (E), I and IV Ly6C⁻F4/80⁺ (F) is shown. CD11b^LoLy6G⁻SiglecF⁻ cells were analyzed for F4/80 and Ly6C expression and five Subpop (I–V) were identified (G). MPO expression in Subpop I F4/80⁺Ly6C^Hi (H), II Ly6C^LoF4/80⁺ (I), III Ly6C⁻F4/80⁻ (J), IV Ly6C⁻F4/80⁺ (K), and V Ly6C⁻F4/80^Hi (L) is shown. The percent positive cells in dot plot gates and the MFI of histograms is provided. Data shown are representative of three mice.