Figure 7.

MLN macrophage subsets differentially express MPO. MLN cells were stained with antibodies that were specific for CD45, CD11b, Ly6C, Ly6G, F4/80, MPO, and SiglecF and using flow cytometry CD45⁺CD11b⁺ cells were analyzed for Ly6G and SiglecF expression to identify Ly6G⁺SiglecF⁻ neutrophils and Ly6G⁻SiglecF⁺ eosinophils (A). Neutrophil expression of MPO is shown (B). CD45⁺CD11b⁻Ly6G⁻SiglecF⁻ cells were analyzed for F4/80 and Ly6C expression and three Subpop (III) were identified (C). Subpop I F4/80⁺Ly6C^Hi cells were separated into CD11b^Hi (1) and CD11b^Lo (2) populations (D), and MPO expression is shown (E and F, respectively). MPO expression in Subpop II Ly6C^LoF4/80⁺ (G) and III Ly6C⁻F4/80⁻ (H) is shown. The percent positive cells in dot plot gates and the MFI of histograms is provided. Data shown are representative of three mice.