Figure 6.

Sex differences in social behaviors and USVs in experiment 2 (different pre-testing isolation). (A–E) Social behaviors and (F–J) USVs of the male and female experimental B6 subjects were assessed by analysis of the video recordings (Observer, Noldus) and spectrograms (Avisoft SASLab Pro), respectively. The B6 female mice were isolated for 3 days in the testing cage before each social encounter as in experiment 1, while the male mice were isolated for only 10 min pre-testing, as in most commonly used procedures. As in experiment 1, social behavior and USVs were analyzed during 3 testing sessions of 3 min each (inter-session interval: 7–10 days) using an adult female CD1 as stimulus. Data are mean ± SEM. N = 10 before exclusion of statistical outliers by Grubb's test for small samples.*p < 0.05. Sex differences are reported as * in each graph legend when a significant main effect of sex was detected in the absence of any interaction with testing session.