Figure 2.

Oxidative PUFA-PL degradation in brain-derived-lipid vesicles to which synthetic SAPC, SDPC, or DMPC has been added. Measurements represent the integrated MRM–LC/MSMS signals of the designated PUFA-PL, divided by the signals from DSPC at each time point, normalized to 1.0 at time zero. Vesicle compositions are listed in Table 2. Lipid vesicle phosphate concentrations were 100 μM, and oxidizing agent concentrations were 500 nM Cu(II) and 50 μM ascorbate. All measurements were performed at room temperature, 21 °C. See methods section for statistical analysis. Left panel: SAPC. Right panel: SDPC.