TABLE 5.
Incorporation of 15N2 into O. officinalis W0012 inoculated with Herbaspirillum sp. strain B501a
| Inoculation | Gas |
15N incorporationb
|
|
|---|---|---|---|
| δ15N (‰) | Atom% excess | ||
| None | Air | 0.9 | <0.001 |
| None | 15N2 | 0.4 | <0.001 |
| B501 | Air | 0.8 | <0.001 |
| B501 | 15N2 | 381 | 0.135 |
a
A gas composed of 40% (vol/vol) 15N2 (99.7 atom%), 40% (vol/vol) Ar, and 20% (vol/vol) O2 was introduced into bottles (120 ml) after several replacements by a gas mixture of 80% (vol/vol) Ar and 20% (vol/vol) O2, or air was introduced into the bottles.
b
After the bottles were inoculated at 30°C for 24 h in the dark, 15N incorporation of rice was determined by mass spectrometry. Values are means of duplicate determinations of each sample.