Figure 3.

Stitch analysis of F59 Fab from a fractionated serum sample. (A) Sequence accuracy of the variable domains of heavy and light chains from a naïve Stitch run (Naïve), compared to a run with selected V-segments for recombination (Refined), added use of nonselected templates as decoys in the final template-matching step (Refined + Decoy), and the synthetic recombinant antibody run with identical Stitch parameters (Recombinant). (B) Sequence alignment of the output sequences (Naïve run excluded for low accuracy) with CDRs annotated and sequence conflicts highlighted by an asterisk (*).