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Drop-plate T-2 toxin sensitivity assay. Twenty-five microliters of ethyl acetate containing 0 (a), 10 (b), 25 (c), 50 (d), or 100 (e) μg of T-2 toxin was spotted onto YEPD-2G plates, and the ethyl acetate was allowed to evaporate. The plates were then spread with 1 × 105 to 2 × 105 conidia and allowed to grow for 2 days.
