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. 2022 Jul 23;12(8):727. doi: 10.3390/membranes12080727

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fluorescence micrographs of binary-component giant unilamellar vesicles (GUVs) that underwent phase separation between the ceramide-rich and ceramide-poor (thus, phospholipid-rich) domains. (A) C16:0SM/C16:0Cer (95:5, molar ratio) GUVs containing 0.2 mol % 594neg-PCer1 (ceramide-rich domain marker) and 0.2 mol % 488neg-SM (SM-rich domain marker). (B) Palmitoyl-oleoyl–phosphatidycholine (POPC)/C16:0Cer (95:5, mole ratio) GUVs containing 0.2 mol % 594neg-PCer1 and BODIPY-PC (POPC-rich fluid phase marker). Bars indicate 10 μm. Image brightness and contrast were adjusted for clarity. This figure was adapted with permission from [41]. Copyright 2019 American Chemical Society.