Figure 4.

AFM images of C16:0SM/Chol/DOPC (2:1:2 molar ratio) bilayers containing 0 (A), 5 (B), and 10 (C) mol % of C16:0Cer. The total mole percentage of SM plus ceramide was kept constant at 40%. All images are 5 × 5 µm with a z-scale of 5 nm. Cross-sections for lines indicated in images (A–C) are shown in a, b, and c, respectively. The Lo domains showed higher membrane thickness while the Ld domains showed lower. Thus, the brighter and darker regions correspond to the Lo and Ld domains, respectively, in these AFM images. Some examples of the convex subdomains for 10% ceramide are indicated by arrows in image C and cross-section c. This figure was adapted with permission from [61]. Copyright 2006 American Chemical Society.