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. 2022 Jul 23;10(8):1779. doi: 10.3390/biomedicines10081779

Figure 1.

Figure 1

Epithelial cell-derived cytokines and chemokines levels during eosinophilic inflammation. (A,B) Innate immune response-associated cytokine mRNA in nasal polyps from patients with ECRS (A) and BEAS-2B cells co-incubated overnight with purified peripheral blood eosinophils or recombinant eosinophil peroxidase (EPX, 10 µg/mL) for 72 h (B). (C) TSLP release from BEAS-2B cells incubated with EPX for 48 h. (D,E) Eosinophil-recruiting chemokines in BEAS-2B cells incubated with TSLP (10 ng/mL) for 48 h, followed by overnight stimulation with EPX (10 µg/mL). CCL4, CCL5, CCL11, and CCL26 mRNA levels (D) and CCL4 release (E) were evaluated. (F) TSLP receptor (TSLPR) mRNA levels in BEAS-2B cells co-incubated with EPX for 48 h. Values in ((A,B,D,F) represent ratios to control (uncinate process tissue; UT from the same patients or non-treatment; vehicle) and values in all panels represent the mean ± standard error of the mean from four experiments; # p < 0.05, ## p < 0.01 (vs. UT or vehicle), * p < 0.05 (as shown between the two groups).