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. 2022 Jul 18;12(12):5574–5595. doi: 10.7150/thno.70754

Figure 1.

Figure 1

UroA and UAS03 chemosensitize the 5FUR colon cancer cells. A. HCT-116-parent and HCT-116-FUR cells (7,000 cells/well) were plated in 96 well plate and grown for overnight. Cells were treated with indicated compounds in a dose dependent manner for 24, 48 and 72 h. The cell viability was measured using standard MTT assay. B. Chemical structures of Urolithin A (UroA) and UAS03. C. HCT-116-FUR cell viability in co-treatments (UroA or UAS03 or 5FU at 50 μM or in combination) was calculated against DMSO (0.1%) as 100%. D. Combination therapy of 5FU with UroA/UAS03 effectively reduced cell proliferation. HCT-116-parental and HCT-116-FUR cells were treated with either 5FU (50 μM), UroA (50 μM), UAS03 (50 μM) or 5FU (50 μM) in combination of UroA (50 μM) or UAS03 (50 μM) for 24 h. Cells were stained with Ki67-Alexa488 and analyzed using flow cytometry. Flow cytometric analysis is represented by histograms and bar diagrams of Ki67+ cells. Data are represented as mean ± S.E.M. of three different experiments. Statistics performed one-way ANOVA using Graph Prism Software. ns: non-significant; ****P < 0.0001.