Skip to main content
. 2022 Jul 18;12(12):5574–5595. doi: 10.7150/thno.70754

Figure 5.

Figure 5

Treatment with UroA/UAS03 regulates the FOXO3-FOXM1 axis. A. Total RNA was isolated from parental HCT-116 and HCT-116-FUR cells and analyzed expression of FOXO3 and FOXM1.The fold changes in mRNA levels were determined by RT PCR method. B. Western blot analysis of FOXO3 and FOXM1in parental HCT-116 and HCT-116-FUR cells. C. Total RNA was isolated from HCT-116-FUR cells treated with either 5FU (50 μM) or UroA (50 μM) or UAS03 (50 μM) or in combination for 24 h and analyzed for the expression of FOXO3, FOXM1 and MRP7 by SyBR RT PCR. The fold changes in mRNA levels were represented. D. HCT-116-FUR cells were treated with either 5FU (50 μM) or UroA (50 μM) or UAS03 (50 μM) or in combination for 24 h. Western blot analysis of FOXO3, FOXM1 and MRP7. Statistics performed one-way ANOVA using Graph Prism Software. Error bars, ±SEM. **p < 0.01, ***p < 0.001.