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. 2022 Jul 18;12(12):5574–5595. doi: 10.7150/thno.70754

Figure 6.

Figure 6

Knockdown of FOXO3 abrogated UroA/UAS03-mediated chemosensitization activities. A. FOXO3 was knockdown in HCT-116-5FUR cells using FOXO3-siRNA. Expression of FOXO3 was confirmed by Western blot. B-E. These cells were treated with either 5FU (50 μM) or UroA (50 μM) or UAS03 (50 μM) or in combination for 24 h and expressions of FOXM1 (B), β-catenin, E-cadherin and BCRP (C) were determined by Western blots. Cell proliferation by Ki67 staining (D) and apoptosis by Annexin V and PI staining using flow cytometry methods (E). Statistics were performed one way ANOVA using Graph Prism Software. Error bars, ±SEM. **p < 0.01, ***p < 0.001.