Figure 7.

UroA/UAS03 co-treatment with 5FU reduce 5FUR tumors in mice. A. Schematic representation of treatment protocol in HCT-116-FUR tumor bearing NRGS mice NRGS mice (n=5-7 per group) were s.c. implanted with HCT-116-FUR and subjected to indicated treatments. B. Change in tumor volume during treatment period. C. Representative gross images of xenograft tumor D-E. Xenograft tumors from dissected mice were measured for tumor volume and tumor weight. F. Total RNA was isolated from tumors and E-cadherin, β Catenin, MDR and BCRP expressions were analyzed. The fold changes in mRNA levels were determined by RT PCR method. G. Protein level expression of E-cadherin, β Catenin, MDR, BCRP, FOXO3 and FOXM1 in the tumors were measured by immunoblots and quantified. Error bars, ±SEM; Statistics performed one way ANOVA using Graph Prism Software. ns: non-significant, ****p < 0.0001; ***p < 0.001; **p < 0.01 *p < 0.05.