Figure 1.

Autophagy flux is inhibited in microglia and infiltrating monocytes in acute phase SCI. Young adult male C57BL/6 mice were subjected to moderate contusion injury at T10 and flow cytometry was used to examine autophagy biomarkers at 3 days post-injury. (A) Representative histograms and mean fluorescent intensity (MFI) quantification show the relative production of autophagosomes in CD45^intCD11b⁺ microglia as measured by the Cyto-ID Autophagy Detection Kit. (B-D) Representative histograms and MFI of p62/SQSTM1 (B), LAMP1 (C), and LAMP2 (D) in CD45^intCD11b⁺ microglia. (E) Representative histograms and MFI of lysosomal activity in microglia as measured by the LysoTracker dye. (F) Representative dot plots and quantitative data depict the composition of infiltrating CD45^hiCD11b⁺ monocytes with Cyto-ID positive staining in the spinal cord injury site. n = 5 mice for Sham group and 9 mice for 3 d SCI group. ***p < 0.001. Two-tailed unpaired t-test.