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. 2022 Jul 11;12(12):5364–5388. doi: 10.7150/thno.72713

Figure 8.

Figure 8

Effects of autophagy deficiency on long-term functional outcome and tissue loss. (A-B) BMS and subscores for WT and Becn1+/- mice at weekly assessment of hindlimb motor function. Two-way repeated measurement ANOVA followed by Holm-Sidak's post-hoc test. n=17 (SCI/WT) and 16 mice (SCI/Becn1+/-). **p < 0.01, ***p < 0.001 vs. SCI/WT. (C-D) Representative images and quantification of spared white matter (SWM) at 6 w post-injury. n=11 (SCI/WT) and 8 mice (SCI/Becn1+/-). *p < 0.05, **p < 0.01 vs. SCI/WT. Two-way ANOVA followed by Tukey's multiple comparison. (E-F) Representative images of GFAP-DAB staining and quantification of the lesion volume at 6 w SCI. n=11 (SCI/WT) and 8 mice (SCI/Becn1+/-). **p < 0.01 vs. SCI/WT. Two-tailed unpaired t-test. (G-H) Representative images of NeuN (purple) staining at the ventral (VH) and dorsal horn (DH) and quantification in the grey matter (I-J) Representative images and quantification of the neurofilament marker SMI312 (red) in the white matter, Scale bars = 100 μm (G) and 50 μm (I). n = 7 (Sham/WT), 5 (Sham/Becn1+/-), 9 (SCI/WT), and 6 (SCI/Becn1+/-) mice/group. ***p < 0.001 vs. Sham groups; #p < 0.05 vs. SCI/WT. Two-way ANOVA followed by Tukey's multiple comparison.