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. 2022 Jul 28;13:377. doi: 10.1186/s13287-022-03023-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Mammary gland development-associated genes’ expression during MLOs formation. A The expression of genes in mammary gland morphogenesis and branching morphogenesis of epithelial tube increased gradually during MLOs generation; B The expression of genes involved in Wnt signaling pathway increased gradually during MLOs formation; C the expression of genes involved in cell division, cell cycle, regulation of transcription and telomere maintenance decreased gradually during MLOs generation. Statistical significance was determined using one-way ANOVA with a Tukey posttest correction. RT-qPCR have been repeated at least three times. Data are depicted as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001