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. 2022 Jul 28;13:377. doi: 10.1186/s13287-022-03023-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Establishing MLOs from human iPSCs is improved by HFF. A Representative bright-field images of MLOs generated from human iPSCs with (lower panel) or without HFF (upper panel) at day 30. Bar, 50 μm. B Branching ratios of MLOs and MLOs-HFF. C Representative immunofluorescence images of MLOs and MLOs-HFF at day 30. Bar, 100 μm. D Sizes of MLOs and MLOs-HFF at different days. Day 10: MLOs (n = 136), MLOs-HFF (n = 107); day 17: MLOs (n = 131), MLOs-HFF (n = 121); day 25: MLOs (n = 83), MLOs-HFF (n = 84); day 30: MLOs (n = 66), MLOs-HFF (n = 78). E Percentage of large organoids (diameter > 200 μm) of MLOs and MLOs-HFF at day 30. F Representative hematoxylin and eosin staining of MLOs and MLOs-HFF at day 30 (left); percentage of organoids displaying duct-like structure in MLOs (n = 13) and MLOs-HFF (n = 39) at day 30 (right). G Representative immunohistochemical staining of MLOs and MLOs-HFF at day 30. Two-tailed Student t test, *p < 0.05, **p < 0.01, ***p < 0.001