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. 2022 Jul 28;11:37. doi: 10.1186/s40035-022-00311-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — RT-QuIC detection of α-syn seeding activity in CSF samples of patients with PD and non-PD. a The average of relative ThT fluorescence from four replicate readings obtained from CSF of each individual cases with PD (n = 24) and non-PD (n = 19) at 80 h. Bars show the average ± SD for each group. The dashed line shows the fluorescence threshold for a positive result. b Traces represent the average percentage of ThT fluorescence readings from positive 22/24 CSF samples from patients with PD and 1/19 non-PD . The means (thick lines) with SD (thin lines) of those averages are shown as a function of RT-QuIC reaction time