Table 1.
Engineered strains generated in this study.
| Strain Name | Overexpressed Genes 1 | Specific Growth Rates in MGYH-glu (h−1) | Specific Growth Rates in MXYH(h−1) | References |
|---|---|---|---|---|
| KM71 | None | 0.07583 ± 0.00066 | 0.00070 ± 0.00008 | Invitrogen |
| PPY0001 | PspXI | 0.07790 ± 0.00099 | 0.00077 ± 0.00005 | This paper |
| PPY0002 | LpXI | 0.07730 ± 0.00108 | 0.00083 ± 0.00005 | This paper |
| PPY0011 | PspXI, PpXKS | 0.05330 ± 0.00106 | 0.00267 ± 0.00005 | This paper |
| PPY0012 | LpXI, PpXKS | 0.06050 ± 0.00128 | 0.00317 ± 0.00005 | This paper |
| PPY0111 | PspXI, PpXKS, PpIlv2, PpIlv6 | 0.05747 ± 0.00094 | 0.00257 ± 0.00008 | This paper |
| PPY0112 | LpXI, PpXKS, PpIlv2, PpIlv6 | 0.05900 ± 0.00179 | 0.00260 ± 0.00008 | This paper |
| PPY0211 |
PspXI, PpXKS, PpIlv5, PpIlv3, LlkivD, ScADH7 |
0.04963 ± 0.00264 | 0.00193 ± 0.00017 | This paper |
| PPY0212 |
LpXI, PpXKS, PpIlv5, PpIlv3, LlkivD, ScADH7 |
0.05813 ± 0.00133 | 0.00287 ± 0.00005 | This paper |
| PPY0311 |
PspXI, PpXKS, PpIlv2, PpIlv6, PpIlv5, PpIlv3, LlkivD, ScADH7 |
0.05097 ± 0.00301 | 0.00260 ± 0.00008 | This paper |
| PPY0312 |
LpXI, PpXKS, PpIlv2, PpIlv6, PpIlv5, PpIlv3, LlkivD, ScADH7 |
0.06153 ± 0.00111 | 0.00280 ± 0.00014 | This paper |
| PPY0100 | PpIlv2, PpIlv6 | 0.07303 ± 0.00264 | 0.00067 ± 0.00005 | This paper |
| PPY0200 | PpIlv5, PpIlv3, LlkivD, ScADH7 | 0.06573 ± 0.00146 | 0.00073 ± 0.00005 | This paper |
| PPY0300 |
PpIlv2, PpIlv6, PpIlv5, PpIlv3, LlkivD, ScADH7 |
0.06330 ± 0.00206 | 0.00083 ± 0.00005 | This paper |
1 PspXI, LpXI, LlkivD, PpIlv2, and PpIlv6 were codon optimized for P. pastoris expression.