Table 1.
Total free thiols and Thioredoxin activity analysis.
| CTRLm | MOLE 1/1000 | MOLE 1/100 | H2O2 | MOLE 1/1000 
H2O2 |
MOLE 1/100 H2O2 |
|
|---|---|---|---|---|---|---|
| TFT a) | 3.69 ± 0.02 | 3.94 ± 0.08 * | 4.07 ± 0.05 * | 3.56 ± 0.03 * | 3.82 ± 0.11 # | 4.03 ± 0.25 # |
| Total Trx b) | 20.46 ± 0.75 | 22.46 ± 0.28 * | 24.31 ± 0.06 * | 17.80 ± 0.77 * | 19.44 ± 1.41 | 21.91 ± 0.80 # |
| Active Trx b) | 3.41 ± 0.17 | 4.76 ± 0.71 * | 5.59 ± 0.16 * | 1.43 ± 0.24 * | 2.62 ± 0.03 * # | 3.37 ± 0.20 # |
| Active Trx (%) | 16.68 ± 0.21 | 21.15 ± 2.29 | 22.98 ± 0.61 * | 8.04 ± 0.99 * | 13.53 ± 0.83 * # | 15.42 ± 1.47 # |
TFT, total free thiols; Trx, thioredoxin; Active Trx, free active form of Trx. C2C12 myotubes were treated with MOLE stock solution dilutions (1/1000 and 1/100 working solution) or vehicle (methanol) in culture media for 24 h. Then, hydrogen peroxide (1 mM) was added to samples pre-treated with vehicle or MOLE for a further hour. After treatments, cells were lysed, and then lysates were used for biochemical analysis. Data presented are the means ± S.D. of three experiments performed in triplicate. a) nmol -SH/g proteins; b) ng Trx/mg proteins. * p < 0.05 vs. CTRLm; # p < 0.05 vs. H2O2.