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. 2022 Jul 24;11(8):1435. doi: 10.3390/antiox11081435

Table 2.

Enzymatic activity analysis.

CTRLm MOLE 1/1000 MOLE 1/100 H2O2 MOLE 1/1000
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H2O2 		MOLE 1/100
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H2O2
SOD a) 0.35 ± 0.01 0.38 ± 0.01 * 0.44 ± 0.05 * 0.29 ± 0.02 ** 0.35 ± 0.03 # 0.36 ± 0.02 ##
CAT a) 3.46 ± 0.16 3.92 ± 0.23 * 4.04 ± 0.09 * 2.97 ± 0.06 ** 3.61 ± 0.13 ## 3.81 ± 0.27 ##
GPx a) 368.49 ± 27.48 468.20 ± 50.01 * 483.92 ± 31.65 * 312.27 ± 17.17 ** 407.63 ± 16.34 ## 432.13 ± 22.66 * ##
GST a) 144.07 ± 6.54 160.26 ± 6.49 * 168.92 ± 3.75 * 123.31 ± 2.61 ** 150.85 ± 5.74 ## ± 10.94 ##

SOD, superoxide dismutase; CAT, catalase; GPx, glutathione peroxidase; GST, glutathione transferase. C2C12 myotubes were treated with MOLE stock solution dilutions (1/1000 and 1/100 working solution) or vehicle (methanol) in culture media for 24 h. Then, hydrogen peroxide (1 mM) was added to samples pre-treated with vehicle or MOLE for a further hour. After treatments, the cells were lysed and then cell lysates were used for biochemical analysis. Data presented are the means ± S.D. of three experiments performed in triplicate. a) U/mg proteins. * p < 0.05 and ** p < 0.01 vs. CTRLm; # p < 0.05 and ## p < 0.01 vs. H2O2.