Figure 1.

Concentration-dependent responses of satellite glial cells (SGCs) to the ganglionic application of BzATP. (A) Representative images illustrate the fluorescence intensity of SGCs at baseline (Frames (F)1–5, 10 s/frame) and 0–20 s (F6–7) after ganglionic application of BzATP (500 µM) in GFAP-GCaMP6s mice. For illustrative purposes, three regions of interest (ROIs) are marked with colored boxes and numbered. (B) Fluorescence intensity traces (F/F₀) of the ROIs shown in (A) before and after the BzATP (500 µM) treatment. Fluorescence intensity was measured for each frame (10 s/frame) and plotted against time or frame number. (C) SGC responses in GFAP-GCaMP6s mice were quantified by measuring the averaged fluorescence intensity in the whole imaging field before (F₀) and after (F) drug treatment. Fluorescence intensity was measured for each frame and plotted against time after ganglionic application of BzATP (5, 50, 500 µM, n = 6 mice/dose). The red arrow indicates the time of drug application. Data are expressed as mean ± SEM. *** p < 0.001 versus vehicle (0 µM); ### p < 0.001 versus indicated group. Two-way repeated measures ANOVA.