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Podoplanin, CD200, and BST2 impact macrophage adhesion to primary LN LECs. (a–d) In vitro adhesion of macrophages to a LN LEC monolayer after podoplanin deletion (a) or antibody-mediated blockade of CD200 (b), BST2 (c), or tenascin (d) measured by flow cytometry. One representative experiment (of 3) is shown with the number of adhered macrophages as percentage of input (N = 4–5), mean, and SD. Ns p ≥ 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, unpaired t-test.
