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. 2022 Jul 23;23(15):8126. doi: 10.3390/ijms23158126

Figure 5.

Figure 5

Ki-67 immunostaining of lung carcinoid grafted embryos. Representative images of 48 hpi Tg(fli1a:EGFP)y1 zebrafish embryos at the level of the graft regions after immunofluorescence assay to detect Ki-67 localization (red). Embryos were implanted with NCI-H835 (a–c), UMC-11 (d–f), NCI-H727 (g–i), and NCI-H720 (j–l) cells. Injected lung carcinoid cells were previously blue-stained (b,e,h,k). The merge of red and blue channels (c,f,i,l) showed that Ki-67 staining is tumor cell specific. Scale bar: 50 µm.