Figure 4.

Cocoa decreased the production of ROS in senescence auditory cells. Representative images of fluorescence micrographs of (A) HEI-OC1, (C) OC-k3 and (E) SV-k1 cells (×40). ROS detection with the fluorescent probe dihydroethidium, DHE (red fluorescent), and DAPI (a marker of cell nuclei with blue fluorescent) dye in cell cultures at the end of the experiments. Experimental groups: CTR group (0; 0.5 and 5 μg/mL of CPE) and H₂O₂ group (induced cellular senescence with 100 µM for 1 h in all groups: 0; 0.5 and 5 μg/mL of CPE).Quantification of fluorescence intensity for DHE probe by ImageJ with (B) HEI-OC1, (D) OC-k3 and (F) SV-k1 cells. The diagrams represent the mean ± SD of Arbitrary units (AU). * p < 0.05 vs. CTR group; # p < 0.05 vs. H₂O₂ group (n = 4).